Mehdi Vafaye Valleh; nahid Karimi zandi; Mahdeieh Najmadini
Volume 21, Issue 2 , July 2019, , Pages 193-207
Abstract
In this research, the effect of in ovo injection of egg yolk of native kahazak chicken into the egg yolk of the commercial Ross 308 strain at prior to incubation on some metabolic-related parameters were tested in the resulting progeny. The search parameters included feed intake, feed conversion ratio, ...
Read More
In this research, the effect of in ovo injection of egg yolk of native kahazak chicken into the egg yolk of the commercial Ross 308 strain at prior to incubation on some metabolic-related parameters were tested in the resulting progeny. The search parameters included feed intake, feed conversion ratio, some of serum biochemical parameters, malondialdehyde concentration as well as relative expression of AMP-activated protein kinase (AMPK) and Neuropeptide Y (NPY) genes in progeny's brain tissue. To achieve this goal, 320 fertile Ross 308 eggs were equally allocated in a completely randomized design into two experimental groups, those injected with Khazak yolk (Test) and control group. Blood and tissue samples were collected at the end of experiment and evaluated for the parameters noted. Compared with the control group, khazak egg-yolk injection resulted in a significant improvement in the feed conversion ratio as well as significant decreases in serum triglyceride, triglyceride/HDL-C ratio, relative visceral fat mass content and levels of AMPK expression in the progeny's brain. In contrast, Khazak yolk injection had no effect on the 45-day body weight, cumulative feed intake, relative brain weight, serum glucose, cholesterol and LDL-c levels but also had no effects on malondialdehyde concentration and NPY expression in progeny brain tissue. According to the results of this study, the injection of native Khazak egg yolk into the yolk of the commercial Ross 308 eggs have effectively improved the feed efficiency and reduced AMPK expression in the diencephalon region of the progeny's brain.
mehdi Vafaye valleh; nahid Karimi zandi; Farzaneh Bazmandegan Shomeyli
Volume 20, Issue 4 , February 2019, , Pages 513-525
Abstract
In this study, effects of the in ovo injection of Khazak egg yolk into the yolk of the Ross 308 eggs on some of offspring's inflammatory and immune indices as well as on the relative expression of intestinal and hepatic TNF-α and/or Zo-1 genes, were investigated. For this purpose, 250 fertile Ross ...
Read More
In this study, effects of the in ovo injection of Khazak egg yolk into the yolk of the Ross 308 eggs on some of offspring's inflammatory and immune indices as well as on the relative expression of intestinal and hepatic TNF-α and/or Zo-1 genes, were investigated. For this purpose, 250 fertile Ross 308 eggs were randomly assigned into two equal experimental groups including test (In ovo injection of Khazak yolk) and control (In ovo injection of Ross yolk) group. After hatching, chickens were reared for six weeks under the same standard environmental conditions with exposure to some certain inflammatory stimuli between 21-28 days of age. Chicken’s blood and/or tissues samples were collected on days 10 and 42, and the samples were analyzed for the target traits. Results showed that Khazak yolk component caused a reduction in the levels of pro-inflammatory TNF-α cytokine in both offspring's liver and intestinal tissue (P<0.05). Compared to the control group, khazak yolk injection was found to enhance the titer of IgA natural antibodyas well as primary and secondary antibody titer response against sheep red blood cells (SRBC) and reduces both serum levels of offspring's CRP protein and liver ALT enzyme (P<0.05). According to the results of the present study, injection of Khazak native hen egg-yolk into the yolk of eggs from Ross 308 commercial broiler breeder can effectively suppress the expression of TNF-α inflammatory cytokine in the offspring's liver and intestinal tissue.